Understanding Phage Therapy

Phage Therapy May Solve The Antibiotic Resistance Crisis… So Why Isn’t It DFA-Approved?

Did you know that the common cold may be lethal once again? It will be if we keep using our current treatments. My learn article focuses on a different approach, phage therapy. Read about what phage therapy is and the obstacles that stand in the way of its FDA approval in Canada and the US.

Modelling Phage Therapy Dynamics Using Python Simulations

Using ChatGPT to generate Python codes, I ran three different simulations monitoring the final population totals of three variables (Phage A, Phage B, and Bacterial Strain X (E. Coli)) after a specific amount of time interacting with each other to showcase phage-bacteria interactions to gain a better understanding of phage therapy dynamics. This worked by setting different parameters for each of those variables that would vary by simulation, with those parameters being legit characteristics of phages or bacteria that professionals would evaluate when determining what phage therapy should be administered to what specific illness or bacteria.

Research + Findings

  • Phage Therapy Focus Presentation

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  • How Does Phage Therapy Actually Work? Modelling Phage-Bacteria Interactions With Python Simulations

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  • Modelling Phage Therapy Dynamics Using Python Simulations

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Methods and Applications for Genetically Engineering Phages to Further Phage Therapy Progress and Combat Obstacles for Clinical Use

To further gain an understanding of the possibilities of genetically engineering phages, I read 9 papers and wrote a review paper on the methods to genetically engineer phages as well as their applications. This paper covers six of the most common methods that researchers/scientists use, as well as the kinds of edits that can be made to phage genomes such as increased host compatibility, increased phage stability in various environments, the deletion of unnecessary genes to minimize phage genomes, and the insertion/modifications various genes for different purposes.

Research + Findings

  • Methods and Applications for Genetically Engineering Phages to Further Phage Therapy Progress and Combat Obstacles for Clinical Use

    Read Here

  • Methods and Applications to Genetically Engineer Phages

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A Phage-Delivered CRISPR-Cas3 System to Target and Suppress the Essential Genes of E. coli CFT073

This CRISPR-Cas3 system will be able to target and gene edit the essential genes of E. coli CFT073 to make the bacterium non-functional. The CRISPR system will be delivered to the bacterium with a gene-edited phage that will carry the CRISPR system. By having all phages gene-edited to carry the same CRISPR system, it creates consistency in phage therapy products.

Research + Findings

  • Phage Therapy Showcase Presentation

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